Most neglected venomous animals belong to the smaller fraction of metazoan life forms, thus their venom yields limited amounts of toxins. Traditional approaches for Biodiscovery are largely pharmacology-driven, which means that venoms were subjected into chromatographic platforms, fractionated and tested. However this approach is rather sample-expensive and most neglected venomous organisms do not deliver required venom yields. Therefore, I apply biotechnological approaches to produce toxins identified in my other projects as an alternative. I utilize chemical synthesis, prokaryote plus eukaryote cell-free systems and recombinant expression strategies in E.coli via different strategies to produce otherwise untestable toxins in large quantities. Moreover, I apply bioengineering approaches to design artificial toxins, either as pharmacologically enhanced derivatives or for structure-function studies, from natural templates and express them alongside.